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RAW 264.7

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Pack Size Availability Purity Price User Price Quantity
1×10⁶cells/T25 1-3days
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Basic information
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Storage Buffer
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Clone Number
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Chemical Name RAW 264.7
Remark RAW 264.7细胞源自Abelson鼠科白血病病毒诱导的肿瘤;sIg-、Ia-抗原、Thy-1.2表面抗原阴性。RAW 264.7细胞不分泌可检测到的病毒颗粒,XC斑点形成试验阴性。RAW 264.7细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖,并且可以抗体依赖性地分解绵羊红血球与肿瘤靶细胞。LPS或PPD处理2天可诱导RAW 264.7细胞分解红血球,但对肿瘤靶细胞无作用。
Storage Instructions 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮
Cell Name RAW 264.7
Biological information
Growth Medium DMEM+10% FBS+1% P/S
Species Reactivity
Growth 贴壁细胞,不用胰酶消化
Antigen Exp H-2d
Applications
Conjugation
Clone
Storage Condition
Gene Exp lysozyme, H-2d; Tested and found negative for ectromelia virus (mousepox).
Doubling Time ~12-30 hours
Receptor Exp complement (C3)
Immunogen
Host Species
Generation Ratio 1:6-1:8
Characters 不规则形
Security information
Culture Conditions 气相:空气,95%;CO2,5%, 温度:37℃
BSL BSL-2
Notes 1.该细胞不建议使用胰酶消化,胰酶会刺激分化;2.超过3天不传代细胞容易分化;3.培养时,存在少量分化细胞,属于正常现象。
Liquid Frequency 2-3次/周
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Publish Date:July 8, 2024
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